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detecting SFSs from short-read unitigs

Open dcopetti opened this issue 3 years ago • 4 comments

Hello, I wonder if the method to detect SFS strings could be applied to other highly accurate datasets such as unitigs created from Illumina reads (e.g. PE, 2x150 bp, 500 bp insert size). In the preprint you mention you use BCALM2 to produce unitigs (from k-mers, OK), but I wonder if any type of "long" (>500 bp?) sequence with very little errors will also result in SFs like the (corrected) HiFi reads. Or does PingPong need to have read redundancy (i.e. HiFi read coverage) to detect SFSs? I wonder if for example the reference can be an assembly/unitigs and the query HiFi reads, and such. Thanks,

Dario

dcopetti avatar Apr 07 '21 10:04 dcopetti