parameters for viral genomes

[YiJessePi]

I was wondering if anyone had the opportunity to test parameters for computing ANI between viral genomes. I guess that the defaults of fragment length of 3000 and minimal number of fragments of 50 is too conservative for small genomes.

[boweny920]

I too have this question. When i tried running 2 exact same viral contig fna files, the output is none, indicating an ANI<80%, which obviously is incorrect.

[YiJessePi]

It might be because you have less fragments than the minimal number of fragments cutoff. The latest version of fastANI use minimal fraction of fragments instead (and I also recommend to reduce the fragments length a bit)

[boweny920]

It might be because you have less fragments than the minimal number of fragments cutoff. The latest version of fastANI use minimal fraction of fragments instead (and I also recommend to reduce the fragments length a bit)

This worked for me! Thanks much!