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krakendb/taxDB and krakendb/taxDB.
Hi aMeta team!
Thanks for providing such a great ancient metagenome pipe. I found a very minor issue when downloading the krakendb from https://doi.org/10.17044/scilifelab.20518251. After wget, there was a 'taxDB.' file and when I run KrakenUniq step, the error log showed:
krakenuniq --preload --db /path/to/resources/krakendb --fastq-input results/CUTADAPT_ADAPTER_TRIMMING/PL0245.trimmed.fastq.gz --threads 8 --output results/KRAKENUNIQ/PL0245/sequences.krakenuniq --report-file results/KRAKENUNIQ/PL0245/krakenuniq.output --gzip-compressed --only-classified-out NOTE: No need to use --gzip-compressed or --bzip2-compressed anymore, format is detected automatically. NOTE: No need to use --fasta-input or --fastq-input anymore, format is detected automatically. Taxonomy database not at /path/to/resources/krakendb/taxDB - creating it .../ path/to/tools/aMeta/resources/krakendb/taxonomy/nodes.dmp does not exist! at /path/to/tools/aMeta/.snakemake/conda/247838bd5e853115f218d1a062fdc248_/bin/krakenuniq line 209.
Krakenuniq works after I changed the name of 'krakendb/taxDB.' to 'krakendb/taxDB'
Guess it could be a mismatch between the downloaded file name and the real name recognized by Krakenuniq code. Hope it would help if any of you has the same problem. Thanks!
jiajun