UnicodeEncodeError: 'ascii' codec can't encode character '\u21b5' in position 4397: ordinal not in range(128)

[yuemo98]

Hi, thanks for your tools!

I am running dRep v3.4.0 for dereplicating the genome, and I have installed the checkm, here is the err. Why does this happen?

---

..:: dRep dereplicate Step 1. Filter ::..

---

Will filter the genome list 3 genomes were input to dRep Calculating genome info of genomes Traceback (most recent call last): File "/home/iozac/huangy1/miniconda2/envs/metagenomic/bin/dRep", line 32, in Controller().parseArguments(args) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/controller.py", line 100, in parseArguments self.dereplicate_operation(**vars(args)) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/controller.py", line 48, in dereplicate_operation drep.d_workflows.dereplicate_wrapper(kwargs['work_directory'],**kwargs) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/d_workflows.py", line 29, in dereplicate_wrapper drep.d_filter.d_filter_wrapper(wd, genomes = genomes, Chdb = Chdb, **kwargs) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/d_filter.py", line 73, in d_filter_wrapper Gdb = calc_genome_info(bdb['location'].tolist()) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/d_filter.py", line 275, in calc_genome_info table['length'].append(calc_fasta_length(loc)) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/drep/d_filter.py", line 655, in calc_fasta_length for seq_record in SeqIO.parse(fasta_loc, "fasta"): File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/Bio/SeqIO/Interfaces.py", line 74, in next return next(self.records) File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/Bio/SeqIO/FastaIO.py", line 206, in iterate Seq(sequence), id=first_word, name=first_word, description=title, File "/home/iozac/huangy1/miniconda2/envs/metagenomic/lib/python3.8/site-packages/Bio/Seq.py", line 1725, in init self._data = bytes(data, encoding="ASCII") UnicodeEncodeError: 'ascii' codec can't encode character '\u21b5' in position 4397: ordinal not in range(128)

Thanks!

[MrOlm]

It seems like your input genomes may be compressed. The only compression supported by dRep is gzip compression, and if you do that the genome filename must end in .gz

[yuemo98]

my input genomes do not compressed, and the genome filename end in .fa

[MrOlm]

This error is occurring because at least one of the files you are including as an input to dRep is not encoded by normal ASCII characters. Maybe you're accidentally including a non-genome file in your input command?