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Double tab in Counts.txt
Hi!
I've noticed in the Counts.txt, unplaced sRNA have double tab between the main and first condition column.
UUUUAUUAUGAUCCAUUUCGCGUGGAAUUCUGGGUGCCAAGGAACUCCAG NA 114 37 15 37 25
In this case, between 114 and 37. What I had to do to solve this was:
tr -s '\t' '\t' < counts.csv > counts.fixed.csv
This could lead to some issues when doing downstream analysis.
Regards!
Thanks for leaving an easy solution! I was also having troubles with it
Yikes. I will make sure this gets fixed in the next major release. Actually I think reporting the unplaced sRNAs in the same file as the locus counts was poor decision in the first place, and I intend to separate them in the next major release.
A new major update to ShortStack, version 4.0, is now in alpha testing: It is available on the ShortStack4 branch. Once we complete alpha testing this will be merged to master and released. But for now you are welcome to test it out. This glitch in the Counts.txt file has been fixed .. the unplaced sRNAs have been removed altogether.