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how to reserve the U&P reads of alignment bam file?

Open Yuzhang0102 opened this issue 8 months ago • 1 comments

Hi Mike, I used ShortStack4 aligned my sRNA reads to genome, and I got the alignment_details.tsv and many bam files. And I have annotation file predicted by other softwares, so I want to call counts using bam and annotation file by featureCounts.

But I need to filter reads that were labelled "R","H","N" in alignment_details.tsv, how can i filter them from bam files?

Thanks a lot.

Yuzhang0102 avatar Jun 05 '24 02:06 Yuzhang0102