Markus Piotrowski

@mdehoon Actually, my discussion was not about the `molecular_weight` function but about `Seq` objects in general. If it wouldn't be better to sanity-check them by default, when they were generated...

@mdehoon `geecee` also ignores `N` for the length calculation or only for the number of G/Cs? There are situations where it may be sensible to guess the GC fraction when...

> Looking at `MeltingTemp` though, it seems that with `strict=True` a `ValueError` is raised if the sequence contains any ambiguous nucleotides, Yes, as a design choice in this special case....

Maybe `strict` as term may be misleading, how about `ignore_ambiguous=True`? I'm not sure if you have already agreed about what 'ignoring' means exactly? So what would `gc_fraction` of 'ACGTN' (with...

> > I'm not sure if you have already agreed about what 'ignoring' means exactly? So what would `gc_fraction` of 'ACGTN' (with ignoring ambiguous bases) be? 0.5 or 0.4? >...

May I add to this discussion? I would be happy to distribute my program as a "one-folder" version on Windows. It should also be part of another software, so an...

@freakboy3742 Thanks for the reply. If I understood you correctly, a `briefcase package -p zip` option is not yet available, right? Actually, it would have helped to have this information...

@freakboy3742 Again, thank you very much for your response. Then I would vote for a `package -p zip` option. Maybe a little background for my comments: I'm not talking about...

@freakboy3742 Please excuse my ignorance about msi installers. Actually, what I wrote was my experience about installers in general, and as you wrote (and I have learned now), msi should...

@ambv I'm sorry, but for me the 'counter-examples' that you show look better/are easier to read than what black is suggesting. Maybe there are different ways how different people read...