Simone Maestri
Simone Maestri
Hi, you may just try setting to 60 GB the RAM memory for that process. It could be that previous processes in the pipeline (together with the OS and some...
Hi, same memory issue as before, but with **assignTaxonomy** process. Please increase also RAM memory for that process, and restart (or try to **-resume**) the pipeline. Edit: I'm not sure...
Hi, since the error is always the same, it means that 60 GB is still not enough for the **assignTaxonomy** process. Please increase further, e.g. to 150 GB, if you...
Closing due to inactivity. Feel free to reopen it, in case yoy have any further issues. Best, SM
Hi, in case you have [seqtk](https://github.com/lh3/seqtk) installed, the following command should work to obtain fastq.gz files with fake quality scores, that you can feed into MetONTIIME. ``` FASTA_DIR="/path/to/dir/with/flye/assemblies" for f...
Hi, please check that the fastq.gz file in **\/downsampleFastq** is not empty and it contains all consensus sequences. As I put a fake quality score for all bases (#), you...
Dear Susie, I just shared with you an example pod5 file referencing this ticket. Best, SM
Hi @sklages, no real solution for the moment. But using the proper bioinformatic tools helps a lot in mitigating such issues. Best, SM
Dear isa, 1. Does the MetONTIIME pipeline address chimeras through the clustering process? Is it worthwhile running my table through something like qiime vsearch uchime-ref or uchime-denovo? In my understanding,...
Hi, closing due to inactivity. Feel free to reopen it, in case you have any further questions. Best, SM