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search_primer_pair fails in the clustalo stage
I'm trying to get a basic working example, using a single primer pair, with the following command:
i=13
search_primer_pair(name=sep_primers$Primer_name[i],
clustal_options=c(exec="D:/Bioinformatics/Tools/R_bins/clustal-omega-1.2.2-win64/clustalo.exe"),
sep_primers$Primer_seq_fw[i], sep_primers$Primer_seq_rv[i],
num_aligns=10, total_primer_specificity_mismatch=3)
But I get the following error:
Error in file(con, "rb") : cannot open the connection
In addition: Warning messages:
1: running command '"D:/Bioinformatics/Tools/R_bins/clustal-omega-1.2.2-win64/clustalo.exe" --infile C:\Users\ibar\AppData\Local\Temp\RtmpqaLDsC\file141c4a4152fb.fas --outfile C:\Users\ibar\AppData\Local\Temp\RtmpqaLDsC\file141cb552430.aln --threads 1' had status 1
2: In file(con, "rb") :
cannot open file 'C:\Users\ibar\AppData\Local\Temp\RtmpqaLDsC\file141cb552430.aln': No such file or directory
It seems like the intermediate fasta file (C:\Users\ibar\AppData\Local\Temp\RtmpqaLDsC\file141c4a4152fb.fas) does not exist in that folder.
Any ideas?
I'm using the latest github version, on windows and R3.3.1.
Thanks, Ido