Run FLAMES directly from an aligned bam file.

[PT806]

Could we run FLAMES directly from a bam file which is generated by other demultiplex tool (i.e. Nanopore/sockeye)? Actually, I have tried once, but failed. It seems that fastq file is required in realign step. Could you please give me some advice if we have no short-read sequencing data but want to use FLAMES for isoform analysis? Thanks so much!