single cell full length RNA-seq mutation detection

[garfield-hu]

hi~

I remember that you use FLAMES to detect mutation and plot the mutation in UMAP. I was so impressed by this part. However I notice that "mutation detection" was not included in sc_long_pipeline.py pipeline and config file while there is did a python script named "bam_mutation.py". I do not know how to use this script, can you provide a tutorial on how you did this ?

thanks

garfield 2021 12 29