synbio icon indicating copy to clipboard operation
synbio copied to clipboard

Published 20 hours ago • verified publisher icon Lattice-Automation

add MAGE

Open jjti opened this issue 4 years ago • 0 comments

Add functionality for oligo design for multiplex automated genome engineering (MAGE).

Existing tools:

  • MODEST (web): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086063/
    • RBS tuning with RBS calculator
    • list of operations lie mutation, substitution, upregulate, knockdown, etc
  • MERLIN (Java): https://www.ncbi.nlm.nih.gov/pubmed/27054880
    • free energy calculation for binding efficiency on primers
    • BLAST to avoid off-target primers
  • OptMAGE (Perl): https://github.com/churchlab/optmage

Picture1

jjti avatar Sep 20 '19 16:09 jjti