Error getDAregion object 'up.clusters' not found

[paybaz]

I am new to DA-seq. I used the dataset from the tutorial to perform DAA. However, at stage 3, when using the getDAregion function, I keep receiving this error: Using min.cell = 50 Error in getDAregion(X = X.melanoma, da.cells = da_cells, cell.labels = X.label.melanoma, : object 'up.clusters' not found

I have to mention that I copied all the lines of code from the tutorial, but the error still shows up.

I would be thankful if someone could resolve this issue for me.

[cfsullivan16]

I had the same issue - it looks like the getDAregion function is written for use with Seurat version 3 or 4. Downgrading to Seurat 4.4.0 and SeuratObject 4.1.4 got rid of this error for me.