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Get multiple graphs (GFA files) with different lengths due to similar depth between mitogenome and plastome

Open nigisensei opened this issue 1 year ago • 5 comments

Dear @Kinggerm I use GetOrganelle to assemble the chloroplast of a species and I got 8 fasta files with different length. The fasta and gfa files is below. I hope you can give me some suggestions to solve this problem. fasta file and gfa file.zip Thank you for the help!

nigisensei avatar Aug 09 '22 01:08 nigisensei

https://github.com/Kinggerm/GetOrganelle/wiki/FAQ#why-are-there-so-many-path_sequencefasta-files

Kinggerm avatar Aug 09 '22 02:08 Kinggerm

Dear @Kinggerm Thank you for your reply! The structure of the GFA files I got is almost the same. Only the total length of the assembled FASTA files is different. The picture is as follows. How can I choose the most appropriate assembled file? BF {V1B94~E02EZV74UQ1KX R73MO2@%I4M2%JD7{ 0%N](https://user-images.githubusercontent.com/94118607/183593866-331c41bc-e059-427f-8052-1651893718be.png) ![N17 SEFE$4410% OGVLT3M](https://user-images.githubusercontent.com/94118607/183593930-e040ee21-9aae-49d6-9b9d-d679bd0102af.png) ![J%CUP79MZDAP$8AKCHK73K ~VF@(PXLK4 7M1J4`BDJW%X

nigisensei avatar Aug 09 '22 07:08 nigisensei

Please visualize the *.fastg file, not the *.gfa file

Kinggerm avatar Aug 09 '22 15:08 Kinggerm

Thank you for your guidance, this is the fastag file after visualization. 1

nigisensei avatar Aug 10 '22 07:08 nigisensei

Please keep the upper-left component and manually remove all other components, then re-visualized the modified graph with name, depth, length, and label from *.csv marked

Kinggerm avatar Aug 10 '22 17:08 Kinggerm