Check using only Pacbio Reads?

[desmodus1984]

Hi, I found your program very interesting and I would like to use to check my pseudohaploid genome. The question that I have is that we only used PacBio reads, and then we used Hi-C to scaffold the assembly. Will it work with only the PacBio reads or do I need both short and long reads?

Thank you

[JiaoLaboratory]

Apologies for the late response. Both single long reads (PacBio/Nanopore) and hybrid approaches combining long and short reads (e.g., Illumina) are acceptable for CRAQ. While the latter （combining long and short reads is preferable for detecting small-scale assembly collapses, it does require more computational resources.