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Hi, Have you seen a similar behavior of picking a threshold using skimage.filters threshold minimum as below before? The threshold automatically set by Scrublet was 0.74 and it seems very...

Hi, If the default assay is either integrated or SCT, should assay="RNA" be set in the "FindAllMarkers" function [here](https://github.com/NHLBI-BCB/IKAP/blob/532b3a2e331179212871b5de162754ecd7e9e614/Seurat3_code/IKAP_Seurat3.R#L157) so that it is not applied to the SCT or integrated...

Is it possible to build a Python applet that uses a python package that requires Python >= 3.6.1? Some of the dependencies like scanpy1.6.0 couldn't be installed. Thanks! J