Johannes Debler

interesting @mbhall88. I'm a bit confused. How did you get barcodes in the duplex bam file? I thought dorado couldn't do duplex calling and barcode assignment at the same time...

Ahhh, ok, that makes sense. Do you do simplex live calling during the run then? We always use post run basecalling, and I don't think that produces the summary file.

That is unfortunate and makes it all the more annoying that guppy doesn't demultiplex the raw files anymore right now (except during live basecalling for some reason....). If that were...

Hi @tijyojwad, just wanted to check if barcode demultiplexing has been integrated into dorado duplex yes, since there have been several releases since October, but I haven't seen anything about...

I found an R script in the [METEORE](https://github.com/comprna/METEORE/blob/master/script/run_megalodon.R) repo that takes the megalodon .bed file and computes methylation frequencies like the nanopolish `calculate_methylation_frequency.py` script does. In its original form the...

Hi @jakezp, I've added your changes to my local install and signed up at openweathermap.org. I keep getthing these errors though: `WARNING 401 Client Error: Unauthorized for url: https://api.openweathermap.org/data/2.5/onecall?lat=XXX&lon=XXX&units=metric&exclude=minutely,hourly,daily,alerts&appid=XXX` On...

Got it, signed up for the free OneCall 3.0 API. Took a few minutes until it was activated and I stopped getting the error. Also, the ow_api.py needs to be...

Hi, not sure if I have the same problem, but running `ufcg profile --input genomes --output output --set NUC --force 1 --thread 10 --metadata metadata.tsv` gives me a `FAILED :...

Hi, I'm getting the same error for another protein: `ERROR! Tree file of gene HEM12 not found : tree/aligned_HEM12_pro.zZ.fasta.treefile` I had a look in the fasta file and there is...

I just ran `tree` with your recent commit version and got this error:  The command used: `ufcg tree -i output_lentis -l label -a nucleotide -t 16 -o output_lentis_tree_nucleotide` Not...