CITE-seq-Count
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Low overlap between cell barcodes and hashtag barcodes
We are trying to demultiplex our samples using Cite-seq but we are getting low overlap between cell barcodes and hashtag barcodes. We used the same pipeline on our previous experiment and didn't have this issue. Here is a summary of our experiment :
Experimental library summary: • Nuclei samples • Nuclei HTO staining • Added HTO cDNA PCR additive primer (V1) • Used 10x dual index plate TT for both HTO library and transcriptome • Not great overlap at demultiplexing step post sequencing
Could you please help us to resolve this issue?
Hey @mary77 just checking that you are the same person I'm talking to via email.
Hey @Hoohm Yes, same person.