phyloFlash
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vsearch error
Hi! Thanks for developing this tool!
I'm getting a somewhat mysterious error that I'm hoping to get some help with! The error occurred on the following subcommand:
running subcommand:
/bioware/vsearch-2.4.0/bin/vsearch -usearch_global
11M.all.final.fasta -id 0.7 -userout 11M.all.vsearch.csv
-userfields
query+target+id+alnlen+evalue+id3+qs+pairs+gaps+mism+ids
-threads 40 --strand plus --notrunclabels -notmatched
11M.all.final.phyloFlash.notmatched.fa -dbmatched
11M.all.final.phyloFlash.dbhits.fa -db
/automounts/workspace/workspace/rweed/Beacon/phyloFlash/138.1/SILVA_SSU.noLSU.masked.trimmed.udb
>11M.all.vsearch.out 2>&1
Error:
FATAL: Tool execution failed!.
Error was '' and return code '256'
Check log file 11M.all.vsearch.out
Check error log file &1
Aborting.
Here is what 11M.all.vsearch.out looks like:
Fatal error: File type not recognized.
It looks like this step should just be inputting a fasta, so it's odd that it's telling me that it doesn't recognize the file type. Any advice would be much appreciated!!