Phyloflash throws error '6400' while running phyloFlash_plotscript_svg.pl

[meet3012]

Hi there,

Firstly, I would like to really appreciate the development of the tool, it has been helping me a lot !!

Recently, while running phyloflash I am getting the below error for some of my input fastas.

I am not able to figure out what's wrong and how I can go about resolving it. Could you please share some thoughts?

I am using the latest version (v3.4.1) and had cloned it directly from the repo (not using anaconda).

Any idea how I could go about the error?

[kbseah]

Hello, thanks for reporting this. Could you please attach the original command you used, the full log, and the output of the log file cami_match.plotscript.out?

Is there a specific reason why you didn't use Conda, such as problems with permissions or wanting to modify the code? If you also share the output of phyloFlash.pl -check_env that would also help.

If you have used a similar command successfully before with other samples, I suspect that this particular read library might have something we didn't anticipate.

[menglemore]

Hi there, has this been solved? I am running into the exact same error (and same message in the .log file) but only for some samples. 3 out of my 8 metagenomic samples encountered this error while the others ran fine.

I downloaded phyloFlash via mamba into its own environment on conda.

Thanks!