Verifybamid2 on long read sequencing data

[reshu23]

Dear Team, I am using verifybamid2 on ONT data. To run verifybamid , at first I convert cram file in to pileup format by using samtools mpileup( with -B option). Then I use pileup as an input to verifybamid2. It is still time taking job. Can I generate pileup format from cram file by using samtools mpileup --positions option? Where I can provide 10K bed file provided in the resource directory to "--positions" parameter. Do you think, it makes sense? Here is the bed file, I am talking about. Thank you.