MPAL-Single-Cell-2019
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Error in lsiProjection
Hi ,
I'm having some trouble with the umapProjection from
'Update scRNA_02_Cluster_Disease_w_Reference_v1.R'
at line 366:
umapProjection <- uwot::umap_transform(as.matrix(lsiProjection$matSVD)[,1:25], umapManifold, verbose = TRUE)
The error is
Error in lsiProjection$matSVD : $ operator not defined for this S4 class
but I cannot see the matSVD slot here, not even within lsiProjection@ .
As I understand, it should be coming from
lsiProjection <- projectLSI(matProjectLSI, lsiReference)
I would like to use it to project a second scRNAseq dataset on a previously determined scRNAseq umap.
Thank you for any comments and tips!
Best, Peter