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Published
20 hours ago •
GreenleafLab
GreenleafLab
Easy bug fix for addClusters to support Leiden igraph implementation
Describe the bug Currently, the addClusters parameter 'method' which can be specified as Seurat or Scran, is the same parameter name as FindClusters parameter method, which is used to specify whether the matrix or igraph method should be used for running leiden (defaults to matrix which is fast for small datasets). It is recommended to set method = 'igraph' to avoid casting large data to a dense matrix, as R does not support long vectors. If you simply renamed the parameter 'method', you could avoid this parameter conflict, allowing us to use the Leiden algorithm, which has been widely adopted in single-cell analysis.
Session Info
R version 4.3.2 (2023-10-31)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 22.04.4 LTS
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.20.so; LAPACK version 3.10.0
Random number generation:
RNG: L'Ecuyer-CMRG
Normal: Inversion
Sample: Rejection
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C LC_TIME=en_US.UTF-8
[4] LC_COLLATE=en_US.UTF-8 LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C LC_ADDRESS=C
[10] LC_TELEPHONE=C LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
time zone: Etc/UTC
tzcode source: system (glibc)
attached base packages:
[1] parallel stats4 grid stats graphics grDevices utils datasets methods base
other attached packages:
[1] uwot_0.1.16 nabor_0.5.0 viridis_0.6.5
[4] viridisLite_0.4.2 UpSetR_1.4.0 TFBSTools_1.40.0
[7] SoupX_1.6.2 SLOcatoR_0.0.0.9000 SingleR_2.4.1
[10] Signac_1.13.0 SeuratWrappers_0.3.5 SeuratDisk_0.0.0.9021
[13] SeuratData_0.2.2.9001 Seurat_5.0.1 SeuratObject_5.0.1
[16] sp_2.1-3 sceasy_0.0.7 scDblFinder_1.16.0
[19] SingleCellExperiment_1.24.0 scales_1.3.0 rstatix_0.7.2
[22] RSQLite_2.3.5 RColorBrewer_1.1-3 patchwork_1.2.0
[25] JASPAR2024_0.99.6 BiocFileCache_2.10.2 dbplyr_2.4.0
[28] infercnv_1.18.1 HCATonsilData_1.0.0 harmony_1.2.0
[31] ggrepel_0.9.5 ggpubr_0.6.0 ggbeeswarm_0.7.2
[34] ggalluvial_0.12.5 GAMBLR.data_1.1.1 EnsDb.Hsapiens.v86_2.99.0
[37] ensembldb_2.26.0 AnnotationFilter_1.26.0 GenomicFeatures_1.54.4
[40] AnnotationDbi_1.64.1 edgeR_4.0.16 limma_3.58.1
[43] ComplexHeatmap_2.18.0 circlize_0.4.16 chromVAR_1.24.0
[46] BiocParallel_1.36.0 Azimuth_0.5.0 shinyBS_0.61.1
[49] BSgenome.Hsapiens.UCSC.hg38_1.4.5 BSgenome_1.70.2 rtracklayer_1.62.0
[52] BiocIO_1.12.0 Biostrings_2.70.3 XVector_0.42.0
[55] lubridate_1.9.3 forcats_1.0.0 dplyr_1.1.4
[58] purrr_1.0.2 readr_2.1.5 tidyr_1.3.1
[61] tibble_3.2.1 tidyverse_2.0.0 reticulate_1.35.0
[64] rhdf5_2.46.1 SummarizedExperiment_1.32.0 Biobase_2.62.0
[67] MatrixGenerics_1.14.0 Rcpp_1.0.12 Matrix_1.6-5
[70] GenomicRanges_1.54.1 GenomeInfoDb_1.38.8 IRanges_2.36.0
[73] S4Vectors_0.40.2 BiocGenerics_0.48.1 matrixStats_1.2.0
[76] data.table_1.15.0 stringr_1.5.1 plyr_1.8.9
[79] magrittr_2.0.3 ggplot2_3.5.0 gtable_0.3.4
[82] gtools_3.9.5 gridExtra_2.3 ArchR_1.0.2
loaded via a namespace (and not attached):
[1] igraph_2.0.2 ica_1.0-3 plotly_4.10.4
[4] scater_1.30.1 zlibbioc_1.48.2 tidyselect_1.2.0
[7] bit_4.0.5 doParallel_1.0.17 clue_0.3-65
[10] lattice_0.21-9 rjson_0.2.21 blob_1.2.4
[13] S4Arrays_1.2.1 caret_6.0-94 seqLogo_1.68.0
[16] png_0.1-8 cli_3.6.2 ProtGenerics_1.34.0
[19] goftest_1.2-3 gargle_1.5.2 bluster_1.12.0
[22] BiocNeighbors_1.20.2 curl_5.2.0 mime_0.12
[25] evaluate_0.23 leiden_0.4.3.1 coin_1.4-3
[28] stringi_1.8.3 pROC_1.18.5 backports_1.4.1
[31] rjags_4-15 parallelDist_0.2.6 XML_3.99-0.16.1
[34] httpuv_1.6.14 rappdirs_0.3.3 splines_4.3.2
[37] RcppRoll_0.3.0 prodlim_2023.08.28 DT_0.32
[40] sctransform_0.4.1 DBI_1.2.2 HDF5Array_1.30.1
[43] withr_3.0.0 class_7.3-22 xgboost_1.7.7.1
[46] lmtest_0.9-40 formatR_1.14 BiocManager_1.30.22
[49] htmlwidgets_1.6.4 fs_1.6.3 biomaRt_2.58.2
[52] labeling_0.4.3 SparseArray_1.2.4 cellranger_1.1.0
[55] annotate_1.80.0 zoo_1.8-12 JASPAR2020_0.99.10
[58] knitr_1.45 TFMPvalue_0.0.9 timechange_0.3.0
[61] foreach_1.5.2 fansi_1.0.6 caTools_1.18.2
[64] timeDate_4032.109 R.oo_1.26.0 poweRlaw_0.80.0
[67] RSpectra_0.16-1 irlba_2.3.5.1 fastDummies_1.7.3
[70] ellipsis_0.3.2 lazyeval_0.2.2 yaml_2.3.8
[73] phyclust_0.1-34 survival_3.5-7 SpatialExperiment_1.12.0
[76] scattermore_1.2 BiocVersion_3.18.1 crayon_1.5.2
[79] RcppAnnoy_0.0.22 progressr_0.14.0 later_1.3.2
[82] ggridges_0.5.6 codetools_0.2-19 base64enc_0.1-3
[85] GlobalOptions_0.1.2 KEGGREST_1.42.0 Rtsne_0.17
[88] shape_1.4.6.1 Rsamtools_2.18.0 filelock_1.0.3
[91] pkgconfig_2.0.3 xml2_1.3.6 GenomicAlignments_1.38.2
[94] spatstat.sparse_3.0-3 ape_5.7-1 xtable_1.8-4
[97] car_3.1-2 fastcluster_1.2.6 httr_1.4.7
[100] tools_4.3.2 globals_0.16.2 hardhat_1.3.1
[103] beeswarm_0.4.0 broom_1.0.5 nlme_3.1-163
[106] futile.logger_1.4.3 lambda.r_1.2.4 hdf5r_1.3.9
[109] ExperimentHub_2.10.0 shinyjs_2.1.0 digest_0.6.34
[112] farver_2.1.1 tzdb_0.4.0 reshape2_1.4.4
[115] ModelMetrics_1.2.2.2 rpart_4.1.21 DirichletMultinomial_1.44.0
[118] glue_1.7.0 cachem_1.0.8 polyclip_1.10-6
[121] generics_0.1.3 mvtnorm_1.2-4 googledrive_2.1.1
[124] presto_1.0.0 parallelly_1.37.0 pkgload_1.3.4
[127] statmod_1.5.0 RcppHNSW_0.6.0 ScaledMatrix_1.10.0
[130] carData_3.0-5 pbapply_1.7-2 spam_2.10-0
[133] dqrng_0.3.2 utf8_1.2.4 gower_1.0.1
[136] ggsignif_0.6.4 lava_1.7.3 shiny_1.8.0
[139] GenomeInfoDbData_1.2.11 R.utils_2.12.3 rhdf5filters_1.14.1
[142] RCurl_1.98-1.14 memoise_2.0.1 rmarkdown_2.25
[145] R.methodsS3_1.8.2 googlesheets4_1.1.1 future_1.33.1
[148] RANN_2.6.1 Cairo_1.6-2 spatstat.data_3.0-4
[151] rstudioapi_0.15.0 cluster_2.1.4 spatstat.utils_3.0-4
[154] hms_1.1.3 fitdistrplus_1.1-11 munsell_0.5.0
[157] cowplot_1.1.3 colorspace_2.1-0 rlang_1.1.3
[160] DelayedMatrixStats_1.24.0 sparseMatrixStats_1.14.0 ipred_0.9-14
[163] dotCall64_1.1-1 shinydashboard_0.7.2 scuttle_1.12.0
[166] xfun_0.42 coda_0.19-4.1 TH.data_1.1-2
[169] CNEr_1.38.0 recipes_1.0.10 remotes_2.4.2.1
[172] iterators_1.0.14 modeltools_0.2-23 abind_1.4-5
[175] interactiveDisplayBase_1.40.0 libcoin_1.0-10 Rhdf5lib_1.24.2
[178] futile.options_1.0.1 bitops_1.0-7 promises_1.2.1
[181] sandwich_3.1-0 DelayedArray_0.28.0 GO.db_3.18.0
[184] compiler_4.3.2 prettyunits_1.2.0 beachmat_2.18.1
[187] listenv_0.9.1 AnnotationHub_3.10.1 BiocSingular_1.18.0
[190] tensor_1.5 MASS_7.3-60 progress_1.2.3
[193] spatstat.random_3.2-2 R6_2.5.1 fastmap_1.1.1
[196] multcomp_1.4-25 fastmatch_1.1-4 vipor_0.4.7
[199] ROCR_1.0-11 nnet_7.3-19 rsvd_1.0.5
[202] KernSmooth_2.23-22 miniUI_0.1.1.1 deldir_2.0-2
[205] htmltools_0.5.7 RcppParallel_5.1.7 bit64_4.0.5
[208] spatstat.explore_3.2-6 lifecycle_1.0.4 restfulr_0.0.15
[211] vctrs_0.6.5 spatstat.geom_3.2-8 scran_1.30.2
[214] future.apply_1.11.1 pracma_2.4.4 pillar_1.9.0
[217] gplots_3.1.3.1 magick_2.8.3 metapod_1.10.1
[220] locfit_1.5-9.8 jsonlite_1.8.8 argparse_2.2.2
[223] GetoptLong_1.0.5
