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Error splitting archr project

Open drighelli opened this issue 1 year ago • 1 comments
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Hi,

I have an ArchR project where I have six samples of two different conditions, but I'm trying to split the project dividing it by condition.

My idea is to select the cells of each condition and then subsetting the project based on these cells, but when I try to do that it returns me a not so much informative error.

Archrlog.log

I tried the following instruction also with dropCells=FALSE and obtained the same error message.

Thanks in advance. Dario

proj_WT <- subsetArchRProject(
  ArchRProj = proj,
  cells = cells,
  outputDirectory = "archr_mo_sleep_WT",
  dropCells = TRUE,
  force=TRUE,
  logFile="ArchRLogs/Archrlog.log"
)
Copying ArchRProject to new outputDirectory :  PATH
Copying Arrow Files...
Getting ImputeWeights
No imputeWeights found, returning NULL
Copying Other Files...
Copying Other Files (1 of 9): Annotations
Copying Other Files (2 of 9): ArchRLogs
Copying Other Files (3 of 9): Embeddings
Copying Other Files (4 of 9): GroupCoverages
Copying Other Files (5 of 9): IterativeLSI
Copying Other Files (6 of 9): PeakCalls
Copying Other Files (7 of 9): Plots
Copying Other Files (8 of 9): Rplots.pdf
Copying Other Files (9 of 9): tmp
Error in saveArchRProject(ArchRProj = ArchRProj[cells, ], outputDirectory = outputDirectory,  : 
  all(file.exists(zfiles)) is not TRUE

Session Info

> sessionInfo()
R version 4.3.1 (2023-06-16)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 22.04.3 LTS
  
Matrix products: default
BLAS:   /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.10.0
LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
  
Random number generation:
 RNG:     L'Ecuyer-CMRG
 Normal:  Inversion
 Sample:  Rejection
  
locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
  
time zone: Etc/UTC
tzcode source: system (glibc)
  
attached base packages:
 [1] parallel  stats4    grid      stats     graphics  grDevices utils
 [8] datasets  methods   base
  
other attached packages:
 [1] rhdf5_2.44.0                SummarizedExperiment_1.30.2
 [3] Biobase_2.60.0              MatrixGenerics_1.12.3
 [5] Rcpp_1.0.11                 Matrix_1.6-0
 [7] GenomicRanges_1.52.0        GenomeInfoDb_1.36.1
 [9] IRanges_2.34.1              S4Vectors_0.38.1
[11] BiocGenerics_0.46.0         matrixStats_1.0.0
[13] data.table_1.14.8           stringr_1.5.0
[15] plyr_1.8.8                  magrittr_2.0.3
[17] ggplot2_3.4.2               gtable_0.3.3
[19] gtools_3.9.4                gridExtra_2.3
[21] ArchR_1.0.2
  
loaded via a namespace (and not attached):
 [1] utf8_1.2.3              generics_0.1.3          bitops_1.0-7
 [4] stringi_1.7.12          lattice_0.21-8          fansi_1.0.4
 [7] scales_1.2.1            abind_1.4-5             cli_3.6.1
[10] rlang_1.1.1             crayon_1.5.2            XVector_0.40.0
[13] munsell_0.5.0           withr_2.5.0             DelayedArray_0.26.7
[16] S4Arrays_1.2.0          tools_4.3.1             dplyr_1.1.2
[19] colorspace_2.1-0        Rhdf5lib_1.22.0         GenomeInfoDbData_1.2.10
[22] vctrs_0.6.3             R6_2.5.1                lifecycle_1.0.3
[25] zlibbioc_1.46.0         pkgconfig_2.0.3         pillar_1.9.0
[28] glue_1.6.2              tibble_3.2.1            tidyselect_1.2.0
[31] rhdf5filters_1.12.1     Cairo_1.6-0             c

drighelli avatar Jan 23 '24 14:01 drighelli