ArchR
ArchR copied to clipboard
Sparse_Marginsum Error Running IterativeLSI
Hello, I'm trying to subset a cell population in mouse samples from my existing ArchR project and re-run clustering/dimensional analysis. I'm able to subset and create a new project but am consistently getting this error at the IterativeLSI step. I'm not too sure how to interpret the R_sparse_marginsum readout, so would really appreciate any insight.
I’ve checked the version of the Matrix package as well (1.6.1-1) so I can’t figure out what’s causing this.
ArchR-addIterativeLSI-3334516593484e-Date-2023-11-21_Time-13-14-39.699038.log
+ ArchRProj = duct2,
+ useMatrix = "TileMatrix",
+ name = "IterativeLSI",
+ iterations = 2,
+ clusterParams = list( #See Seurat::FindClusters
+ resolution = c(0.4),
+ sampleCells = 10000,
+ n.start = 10
+ ),
+ varFeatures = 25000,
+ dimsToUse = 1:5,
+ force = TRUE
+ )
Checking Inputs...
ArchR logging to : ArchRLogs/ArchR-addIterativeLSI-3334516593484e-Date-2023-11-21_Time-13-14-39.699038.log
If there is an issue, please report to github with logFile!
2023-11-21 13:14:40.754279 : Computing Total Across All Features, 0.008 mins elapsed.
2023-11-21 13:14:42.515783 : Computing Top Features, 0.038 mins elapsed.
###########
2023-11-21 13:14:46.103587 : Running LSI (1 of 2) on Top Features, 0.097 mins elapsed.
###########
2023-11-21 13:14:46.138874 : Creating Partial Matrix, 0.098 mins elapsed.
2023-11-21 13:14:54.908973 : Computing LSI, 0.244 mins elapsed.
************************************************************
2023-11-21 13:14:55.197164 : ERROR Found in .computeLSI for
LogFile = ArchRLogs/ArchR-addIterativeLSI-3334516593484e-Date-2023-11-21_Time-13-14-39.699038.log
<simpleError in .local(x, na.rm, dims, ...): object 'R_sparse_marginsum' not found>
************************************************************
************************************************************
2023-11-21 13:14:55.33462 : ERROR Found in .LSIPartialMatrix for
LogFile = ArchRLogs/ArchR-addIterativeLSI-3334516593484e-Date-2023-11-21_Time-13-14-39.699038.log
<simpleError in .logError(e, fn = ".computeLSI", info = "", errorList = errorList, logFile = logFile): Exiting See Error Above>
************************************************************
Error in .logError(e, fn = ".LSIPartialMatrix", info = "", errorList = errorList, :
Exiting See Error Above```