ArchR
ArchR copied to clipboard
GreenleafLab
addGeneIntegrationMatrix fails with tutorial dataset and Seurat 5.0.0
Attach your log file ArchR-addGeneIntegrationMatrix-12d1d6685d430-Date-2023-11-11_Time-17-32-30.524157.log
Describe the bug
In the ArchR tutorial section 8.1 running addGeneIntegrationMatrix fails, with the error:
Error in slot(object = object, name = "features")[[layer]] <- features :
more elements supplied than there are to replace
Running traceback() shows the following function stack:
13: `LayerData<-.Assay5`(object = `*tmp*`, layer = layer, features = features[[layer]],
cells = cells[[layer]], transpose = transpose, value = counts[[layer]])
12: `LayerData<-`(object = `*tmp*`, layer = layer, features = features[[layer]],
cells = cells[[layer]], transpose = transpose, value = counts[[layer]])
11: .CreateStdAssay.list(counts = counts, min.cells = min.cells,
min.features = min.features, transpose = transpose, type = type,
csum = csum, fsum = fsum, ...)
10: .CreateStdAssay(counts = counts, min.cells = min.cells, min.features = min.features,
transpose = transpose, type = type, csum = csum, fsum = fsum,
...)
9: CreateAssay5Object(counts = counts, min.cells = min.cells, min.features = min.features,
...)
8: CreateSeuratObject.default(counts = mat[head(seq_len(nrow(mat)),
5), , drop = FALSE])
7: Seurat::CreateSeuratObject(counts = mat[head(seq_len(nrow(mat)),
5), , drop = FALSE])
6: FUN(X[[i]], ...)
5: lapply(...)
4: .safelapply(seq_along(blockList), function(i) {
prefix <- sprintf("Block (%s of %s) :", i, length(blockList))
.logDiffTime(sprintf("%s Computing Integration", prefix),
tstart, verbose = verbose, logFile = logFile)
blocki <- blockList[[i]]
subProj@cellColData <- subProj@cellColData[blocki$ATAC, ]
subProj@sampleColData <- subProj@sampleColData[unique(subProj$Sample),
, drop = FALSE]
subRNA <- seuratRNA[, blocki$RNA]
subRNA <- subRNA[rownames(subRNA) %in% geneDF$name, ]
.logDiffTime(sprintf("%s Identifying Variable Genes", prefix),
tstart, verbose = verbose, logFile = logFile)
subRNA <- FindVariableFeatures(object = subRNA, nfeatures = nGenes,
verbose = FALSE)
subRNA <- ScaleData(object = subRNA, verbose = FALSE)
if (is.null(genesUse)) {
genesUse <- VariableFeatures(object = subRNA)
}
.logDiffTime(sprintf("%s Getting GeneScoreMatrix", prefix),
tstart, verbose = verbose, logFile = logFile)
...
3: Reduce("rbind", .)
2: .safelapply(seq_along(blockList), function(i) {
prefix <- sprintf("Block (%s of %s) :", i, length(blockList))
.logDiffTime(sprintf("%s Computing Integration", prefix),
tstart, verbose = verbose, logFile = logFile)
blocki <- blockList[[i]]
subProj@cellColData <- subProj@cellColData[blocki$ATAC, ]
subProj@sampleColData <- subProj@sampleColData[unique(subProj$Sample),
, drop = FALSE]
subRNA <- seuratRNA[, blocki$RNA]
subRNA <- subRNA[rownames(subRNA) %in% geneDF$name, ]
.logDiffTime(sprintf("%s Identifying Variable Genes", prefix),
tstart, verbose = verbose, logFile = logFile)
subRNA <- FindVariableFeatures(object = subRNA, nfeatures = nGenes,
verbose = FALSE)
subRNA <- ScaleData(object = subRNA, verbose = FALSE)
if (is.null(genesUse)) {
genesUse <- VariableFeatures(object = subRNA)
}
.logDiffTime(sprintf("%s Getting GeneScoreMatrix", prefix),
tstart, verbose = verbose, logFile = logFile)
...
1: addGeneIntegrationMatrix(ArchRProj = projHeme2, useMatrix = "GeneScoreMatrix",
matrixName = "GeneIntegrationMatrix", reducedDims = "IterativeLSI",
seRNA = seRNA, addToArrow = FALSE, groupRNA = "BioClassification",
nameCell = "predictedCell_Un", nameGroup = "predictedGroup_Un",
nameScore = "predictedScore_Un")
To Reproduce Run the ArchR tutorial steps until Section 8.1. Download the seRNA dataset as described, then run
projHeme2 <- addGeneIntegrationMatrix(
ArchRProj = projHeme2,
useMatrix = "GeneScoreMatrix",
matrixName = "GeneIntegrationMatrix",
reducedDims = "IterativeLSI",
seRNA = seRNA,
addToArrow = FALSE,
groupRNA = "BioClassification",
nameCell = "predictedCell_Un",
nameGroup = "predictedGroup_Un",
nameScore = "predictedScore_Un"
)
which causes the error.
Expected behavior As described in the tutorial.
Session Info
R version 4.3.2 (2023-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Sonoma 14.1.1
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/lib/libRlapack.dylib; LAPACK version 3.11.0
Random number generation:
RNG: L'Ecuyer-CMRG
Normal: Inversion
Sample: Rejection
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
time zone: Europe/London
tzcode source: internal
attached base packages:
[1] stats4 grid stats graphics grDevices utils datasets methods base
other attached packages:
[1] presto_1.0.0 harmony_1.1.0 uwot_0.1.16
[4] nabor_0.5.0 Rsamtools_2.18.0 BSgenome.Hsapiens.UCSC.hg19_1.4.3
[7] BSgenome_1.70.1 rtracklayer_1.62.0 BiocIO_1.12.0
[10] Biostrings_2.70.1 XVector_0.42.0 magick_2.8.1
[13] rhdf5_2.46.0 SummarizedExperiment_1.32.0 Biobase_2.62.0
[16] MatrixGenerics_1.14.0 Rcpp_1.0.11 Matrix_1.6-1.1
[19] GenomicRanges_1.54.1 GenomeInfoDb_1.38.0 IRanges_2.36.0
[22] S4Vectors_0.40.1 BiocGenerics_0.48.1 matrixStats_1.1.0
[25] data.table_1.14.8 stringr_1.5.0 plyr_1.8.9
[28] magrittr_2.0.3 ggplot2_3.4.4 gtable_0.3.4
[31] gtools_3.9.4 gridExtra_2.3 ArchR_1.0.2
[34] Seurat_5.0.0 SeuratObject_5.0.0 sp_2.1-1
loaded via a namespace (and not attached):
[1] RcppAnnoy_0.0.21 splines_4.3.2 later_1.3.1 bitops_1.0-7
[5] tibble_3.2.1 polyclip_1.10-6 XML_3.99-0.15 fastDummies_1.7.3
[9] lifecycle_1.0.4 globals_0.16.2 lattice_0.22-5 MASS_7.3-60
[13] plotly_4.10.3 yaml_2.3.7 httpuv_1.6.12 sctransform_0.4.1
[17] spam_2.10-0 spatstat.sparse_3.0-3 reticulate_1.34.0 cowplot_1.1.1
[21] pbapply_1.7-2 RColorBrewer_1.1-3 abind_1.4-5 zlibbioc_1.48.0
[25] Rtsne_0.16 purrr_1.0.2 RCurl_1.98-1.13 GenomeInfoDbData_1.2.11
[29] ggrepel_0.9.4 irlba_2.3.5.1 listenv_0.9.0 spatstat.utils_3.0-4
[33] pheatmap_1.0.12 goftest_1.2-3 RSpectra_0.16-1 spatstat.random_3.2-1
[37] fitdistrplus_1.1-11 parallelly_1.36.0 leiden_0.4.3 codetools_0.2-19
[41] DelayedArray_0.28.0 tidyselect_1.2.0 farver_2.1.1 spatstat.explore_3.2-5
[45] GenomicAlignments_1.38.0 jsonlite_1.8.7 ellipsis_0.3.2 progressr_0.14.0
[49] ggridges_0.5.4 survival_3.5-7 tools_4.3.2 ica_1.0-3
[53] glue_1.6.2 SparseArray_1.2.2 dplyr_1.1.3 withr_2.5.2
[57] fastmap_1.1.1 rhdf5filters_1.14.1 fansi_1.0.5 digest_0.6.33
[61] R6_2.5.1 mime_0.12 colorspace_2.1-0 scattermore_1.2
[65] Cairo_1.6-1 tensor_1.5 spatstat.data_3.0-3 RhpcBLASctl_0.23-42
[69] utf8_1.2.4 tidyr_1.3.0 generics_0.1.3 httr_1.4.7
[73] htmlwidgets_1.6.2 S4Arrays_1.2.0 pkgconfig_2.0.3 lmtest_0.9-40
[77] htmltools_0.5.7 dotCall64_1.1-0 scales_1.2.1 png_0.1-8
[81] rstudioapi_0.15.0 reshape2_1.4.4 rjson_0.2.21 nlme_3.1-163
[85] zoo_1.8-12 KernSmooth_2.23-22 parallel_4.3.2 miniUI_0.1.1.1
[89] restfulr_0.0.15 pillar_1.9.0 vctrs_0.6.4 RANN_2.6.1
[93] promises_1.2.1 xtable_1.8-4 cluster_2.1.4 cli_3.6.1
[97] compiler_4.3.2 rlang_1.1.2 crayon_1.5.2 future.apply_1.11.0
[101] labeling_0.4.3 stringi_1.7.12 viridisLite_0.4.2 deldir_1.0-9
[105] BiocParallel_1.36.0 munsell_0.5.0 lazyeval_0.2.2 spatstat.geom_3.2-7
[109] RcppHNSW_0.5.0 patchwork_1.1.3 future_1.33.0 Rhdf5lib_1.24.0
[113] shiny_1.7.5.1 ROCR_1.0-11 igraph_1.5.1
Additional context Note that I'm running this on an M1 Mac (as shown in the Session Info above). Any help with further debugging steps I can take would be much appreciated.
Hi @chloeacolson! Thanks for using ArchR! Please make sure that your post belongs in the Issues section. Only bugs and error reports belong in the Issues section. Usage questions and feature requests should be posted in the Discussions section, not in Issues.
It is worth noting that there are very few actual bugs in ArchR. If you are getting an error, it is probably something specific to your dataset, usage, or computational environment, all of which are extremely challenging to troubleshoot. As such, we require reproducible examples (preferably using the tutorial dataset) from users who want assistance. If you cannot reproduce your error, we will not be able to help.
Before going through the work of making a reproducible example, search the previous Issues, Discussions, function definitions, or the ArchR manual and you will likely find the answers you are looking for.
If your post does not contain a reproducible example, it is unlikely to receive a response.
__In addition to a reproducible example, you must do the following things before we help you, unless your original post already contained this information:
1. If you've encountered an error, have you already searched previous Issues to make sure that this hasn't already been solved?
2. Did you post your log file? If not, add it now.
3. Remove any screenshots that contain text and instead copy and paste the text using markdown's codeblock syntax (three consecutive backticks). You can do this by editing your original post.
