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BiocParallel error when using Bambu in quantification mode
Hi Andre and the Bambu team,
I am encountering an error when I am running Bambu in quantification mode on some of my samples. After our discussions in issue #448, I decided to try to include all detected transcripts in my analysis as NDR < 0.4 for all of the transcripts. I am finding however that for some of my samples now I am encountering a BiocParallel which reads:
Error: BiocParallel errors
1 remote errors, element index: 1
0 unevaluated and other errors
first remote error:
Error in if (annotatedIntronNumberNew > annotatedIntronNumber & !is.na(annotatedIntronNumber)) {: missing value where TRUE/FALSE needed
In addition: There were 15 warnings (use warnings() to see them)
The commands I am using to run Bambu are:
annotations <- prepareAnnotations(gtf.file)
create_directory(outdir)
se.quantOnly <- bambu(reads = input_bam,
annotations = gtf.file,
genome = fa.file,
discovery = FALSE,
verbose=TRUE,
lowMemory=TRUE,
yieldSize=1e6)
writeBambuOutput(se.quantOnly, path = outdir)
se_out <- sprintf("%s/se.quantOnly.RData",outdir)
save(se.quantOnly, file = se_out)
I am attaching the full log here in case it is helpful:
This was run with bambu 3.5.1. Any clues as to what's going on here?
Thanks, Asher