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Adjust pc.range in IKAP for SCTransform()-processed Seurat objects?

Open denvercal1234GitHub opened this issue 4 years ago • 6 comments

Dear @NHLBI-BCB and @xizhihui

Thank you for developing the package!

I have processed my data using SCTransform() (which replaces NormalizeData(), ScaleData() and FindVariableFeatures()) and have performed RunPCA, RunUMAP, FindNeighbors() and FindClusters().

SCTransform() allows using 30 PCs, should I adjust pc.range = 30 or just use the default value of 20 and let the IKAP() decides? I am confused because even if the IKAP Package info says pc.range = 20 as default, I still see the Iteration running for nPC = more than 20.

Also, would you mind advising me how to obtain the resolution of a recommended (PC,k) combination at the end?

Thank you very much for your help!

denvercal1234GitHub avatar Feb 21 '21 11:02 denvercal1234GitHub