IKAP
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Adjust pc.range in IKAP for SCTransform()-processed Seurat objects?
Dear @NHLBI-BCB and @xizhihui
Thank you for developing the package!
I have processed my data using SCTransform() (which replaces NormalizeData(), ScaleData() and FindVariableFeatures()) and have performed RunPCA, RunUMAP, FindNeighbors() and FindClusters().
SCTransform() allows using 30 PCs, should I adjust pc.range = 30 or just use the default value of 20 and let the IKAP() decides? I am confused because even if the IKAP Package info says pc.range = 20 as default, I still see the Iteration running for nPC = more than 20.
Also, would you mind advising me how to obtain the resolution of a recommended (PC,k) combination at the end?
Thank you very much for your help!