Augustus
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Get focused on eucaryotic genes with augustus
Hello, I need to predict strict eucaryotic genes from my genome, for that I use Augustus with training parameters used before and I remove gene predicted when they do not have intron in their sequence (typical signature of non-eucaryote sequence).
But it appears that even by doing that Augustus still predicts gene sfrom non-eucaryotic origin ( even with intron in their sequences).
So my question is:
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1- is there a way to be more stingent in order to only catch eucaryotic genes
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2- is there a way to get statistical values giving the probability that a predicted gene is really from eucaryotic origin ?
I saw in the doc that there were two kind of statistics :
POSTERIOR PROBABILITIES
For this one I do not see the posterior probabilities affected to predicted genes , maybe there is a particular obtion to activate?
and
the MEA USING THE MAXIMUM EXPECTED ACCURACY APPROACH
with the option -mae
For this one I do not realy understand, what are we expecting to see in the output file ? Is there a metric wich tells us how much we can be confident about a predicted gene ?
Thank you for your help