Unable to read file magic number

[GaojieLi-dot]

When I use bismark to map DNA methylation data, the log file shows this error. At the same time, the mapping is also very low. error infotmation: Unable to read file magic number is_zstd_file: unable to read magic number Unable to read file magic number is_zstd_file: unable to read magic number Unable to read file magic number is_zstd_file: unable to read magic number

report: Sequences analysed in total: 2401399 Number of alignments with a unique best hit from the different alignments: 772217 Mapping efficiency: 32.2% Sequences with no alignments under any condition: 1435910 Sequences did not map uniquely: 193272 Sequences which were discarded because genomic sequence could not be extracted: 0

Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 176210 ((converted) top strand) CT/GA: 175036 ((converted) bottom strand) GA/CT: 210189 (complementary to (converted) top strand) GA/GA: 210782 (complementary to (converted) bottom strand)

command: bismark -q -N 1 --parallel 5 --non_directional --bam --temp_dir ./ -un ./ --bowtie2 --genome hg38 fastq.gz -o ./