Bismark
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Bismark mapping issue
Hi Developers,
Thanks a lot for providing such a useful tool. When I used the bismark code, I met with a very strange issue that did not appear before. I have three WGBS samples with paired-end sequencing. The size of each R1.fastq.gz is about 30G. I ssh the server three times to process the three samples simultaneously. However, the processes all ended abnormally after about ~60 million sequences were processed. Then I tried to check the generated bam files, however, when I entered the directory harboring the fastq and bam files, "ls" does not give any response and "ctrl+c" does not kill it. Could you please tell me how to fix such an issue?
Bismark version: 0.23.1 bismark --genome /data/REF/hg19 -1 xxx_R1.fastq.gz -2 xxx_R2.fastq.gz