ANI values for the MAGs

[saras224]

Hi @pchaumeil I have a confusion regarding the ANI values that the GTDB-tk gives for the classification of the MAGs.

1. which one should I consider the ANI values of the MAGs with the references; fastani_ani or closest_placement_ani?
2. why does gtdbtk not assigning ANI values to all the MAGs and giving NA instead? is there any cut-off after which the gtdbtk tool does not give the ANI value for the MAGs and gives NA?
3. This one is a general question: if the ANI match with the reference is ~70% then to which classification level it is similar (family or order?
4. if a MAG is novel and it has to be called as new phyla then what should be the ANI match for it?

Hope you understand what my confusion is and you would clarify the doubts.

Thanks in Advance Saraswati Awasthi