DoubletDecon

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Opening DoubletDecon Shiny app

1

Hi, I'm having trouble opening DoubletDecon in Shiny. I've tried both of your suggestions from below. When I try downloading and clicking on DoubletDecon.app I get this error message :...

abigailelliot26

Problem with `filter()` input `..1`

3

Dear Authors, Thanks for creating such a useful tool! I am trying to use this tool with processed data from Seurat object. However, in the Improved_Seurat_Pre_Process I am getting an...

rahulnutron

Hi. Thank you for this great package. Which information from the results of running DoubletDecon output gives me information about which cell in my Seurat data set is a doublet?...

charlesgwellem

the results of Demuxlet dataset

2

Hi, I'm trying to run DoubletDecon using Demuxlet dataset by running 20 times. However, I haven't gotten a result with high accuracy, I'm wondering if there are some errors in...

JuanruMaryGuo

Exporting Seurat object to the DoubletDecon app

1

Hello, I'm very new to programming so I apologise if this is an annoying question. I've used the Improved_Seurat_Pre_Process and would now like to use the DoubletDecon app to first...

Hatfield123

untar issue during installation

2

Hi I'm having trouble with installing doubletdecon, and it seems like R cannot untar the file successfully. 

xcliu-oc

scATAC-seq doublet detection

1

Very useful and easy to use tool! I wanted to ask if DoubletDecon can be repurposed for scATAC-seq doublet detection. Theoretically, you could run DoubletDecon on the **gene activity matrix**...

RegnerM2015

Trouble understanding the output

1

Hi, I have successfully run the tool both in R and in the app. In the app, the barplot shows the number of predicted singlet, rescue singlet and final doublet....

yen-tran-91

DoubletDecon - Main_Doublet_Decon - no locations are finite error.

4

Hi, I'm testing out DoubletDecon 1.1.4 with data which has been processed using Seurat's standard method, and then using the example provided here on the DoubletDecon git. However the results=Main_Double_Decon...

kmshort

Error in paste0(location, filename, "_groups") : cannot coerce type 'closure' to vector of type 'character'

6

Hi, I followed your example starting from per-existing Seurat Object. Function "Improved_Seurat_Pre_Process" executed without any problems. However there is something wrong with the next step (which by the way there...

DirtyHarry80