Classified % went down after I added genomes that should be informative

[dgolden96]

Hi there,

I'm seeing some unexpected results from an update I recently ran on my kraken2 database. I started with a database drawing on sequence data from ~120,000 genomes, and I added about ~5,000 more that should have been very informative for the reads I'm trying to classify, but my updated database is actually classifying an ever-so-slightly smaller percentage of reads than it did before. Only an average decrease of about 0.05% reads classified. Does anyone have any guesses about what might be causing this? I used the Struo2 pipeline to update the database with the new genomes.

Thanks!