u-shape3D
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The image brightness is saturated after doing deconvolution
I want to use data that I have acquired using SPIM. But when I choose deconvolution and mesh, after deconvolution I get that image brightness is saturated and then in the next step (mesh) software freezes. I don´t know what could be the problem. Is there maybe a way to adjust psf file to fit to our set-up? Or is there some other way to deal with this problem? Thank you in advance