Can I use metacell data as a reference?

[JHovelly]

Thank you for your excellent algorithm, I have a question that if I use metacell data, which means a metacell is a sum of dozens of single cells, as a reference, whether the deconvolution result is accurate. Best wishes! J Hovelly.

[tinyi]

Thank you for your interest in our method.

It might be a good idea to use metacell as cell states, to model the heterogeneity, although we have not tested this. However, we discourage the use of metacell for cell types, as we suggest each cell type to have sufficient number of differentially expressed genes to ensure the accuracy, and metacells might be too similar to resolve.

On Sat, Mar 11, 2023 at 7:45 AM JHovelly @.***> wrote:

Thank you for your excellent algorithm,

I have a question that if I use metacell data, which means a metacell is a sum of dozens of single cells, as a reference, whether the deconvolution result is accurate.

Best wishes! researcher from China.

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