Can't find function trans_ordination

[Jgruetzke]

Hello, I'm using microeco version 1.6.0 with R version 4.2.0 to do RDA and dbRDA analysis, but I get an error for trans_ordination: t1$trans_ordination(adjust_arrow_length = TRUE, max_perc_env = 1.5) Error: attempt to apply non-function I also checked for the availability of the function but cannot find it. ls("package:microeco") [1] "clone" "dataset" [3] "dropallfactors" "env_data_16S" [5] "fungi_func_FungalTraits" "fungi_func_FUNGuild" [7] "microtable" "otu_table_16S" [9] "otu_table_ITS" "phylo_tree_16S" [11] "prok_func_FAPROTAX" "prok_func_NJC19_list" [13] "sample_info_16S" "sample_info_ITS" [15] "Tax4Fun2_KEGG" "taxonomy_table_16S" [17] "taxonomy_table_ITS" "tidy_taxonomy" [19] "trans_abund" "trans_alpha" [21] "trans_beta" "trans_classifier" [23] "trans_diff" "trans_env" [25] "trans_func" "trans_network" [27] "trans_norm" "trans_nullmodel" [29] "trans_venn"
I'm very confused about it. Is it my installation. However, I did not have any problem with that.

Thank you very much for your help.

[ChiLiubio]

Hi. How is your t1 object created? Please attach an example with the data in the package. The error message shows there is no trans_ordination function in it. For the help document, please run ?trans_env

[Jgruetzke]

Thank you very much for your help. Indeed, my t1 object wasn't created properly. Is there a limited number of metadata that can be used here? I ran into an error when I tried to use more than 60 metadata for the cal_ordination function.

[ChiLiubio]

I guess the error comes from the dependent function of vegan package. Although there is no number limitation there, it is better to select some important variables.