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Adding intronic and spliced TPM reads?

Open cnk113 opened this issue 5 years ago • 0 comments

Hello,

I was wondering if I can just sum up the intronic TPM reads and spliced TPM reads for a gene for a bulk RNA-seq sample? This is using the mapping-based mode. The index was created following RNA velocity vignette with alevin. I ended up using the same index, and I've found for my scRNA-seq data that adding the spliced and unspliced reads gave me tighter clusters. I would like to the same for my bulk samples as well. I assume due to the TPM normalization I can't just sum it up for a gene?

cnk113 avatar Sep 10 '20 04:09 cnk113