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Library type for Nanopore libraries

Open imet-k opened this issue 6 years ago • 2 comments

I tried to use this tool for Nanopore direct RNA sequencing. However, library type was not recognized.

imet-k avatar Mar 27 '18 06:03 imet-k

I used -l SR (stranded and reverse strand) for nanopore direct RNA Seq data.

sdwien avatar Dec 19 '18 12:12 sdwien

I have used "U" as was default in config.yml for pipeline-transcriptome-de but now I am thinking it should be stranded but i am not understanding the reverse part? Should it not be SF as this is essentially single-read and the baseball sequence is output as 5' to 3' of original RNA molecule despite being sequenced in the reverse from 3' to 5'.

callumparr avatar Apr 19 '21 03:04 callumparr