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Adding support to read three-dimensional somata and build them accurately.

Open marwan-abdellah opened this issue 3 years ago • 7 comments

Received some SWC morphology file from @orena1. The file contains multiple soma contours at different depths-of-field. These contours are being handled for the moment as basal dendrites. We therefore must integrate a utility to identify the presence of 3d somata contours and reconstruct them accurately with metaball algorithm only. I think that the SoftBody simulation might not be that accurate.

marwan-abdellah avatar Jul 28 '20 11:07 marwan-abdellah

@orena1 I am working on implementing the multi-layer soma. Can you provide a sample SWC file with reconstructed soma?

marwan-abdellah avatar Nov 17 '20 17:11 marwan-abdellah

Thanks @marwan-abdellah : tracingSAC1_aftLunch_16.swc.zip

orena1 avatar Nov 17 '20 21:11 orena1

@orena1 The profile is projected on a single plane. Don't you have another one with real 3d profile. 3d-soma-plane

marwan-abdellah avatar Nov 24 '20 19:11 marwan-abdellah

Maybe this: Downloads.zip

The original reconstruction is asc, I transformed it to swc using this website: https://neuroinformatics.nl/HBP/morphology-viewer/

Thanks!

orena1 avatar Nov 25 '20 16:11 orena1

Also this 2017-13-02-slice-2-cell-3-rotated.swc.zip Thanks

orena1 avatar Jan 13 '22 00:01 orena1

@orena1 I was able to load and render the neuron, but I don't even find any contours to the soma. Are you sure they exist? 2017-13-02-slice-2-cell-3-rotated_morphology_front-1 2017-13-02-slice-2-cell-3-rotated_morphology_front-2

Projections with closeup on 2017-13-02-slice-2-cell-3-rotated_morphology_front 2017-13-02-slice-2-cell-3-rotated_morphology_side 2017-13-02-slice-2-cell-3-rotated_morphology_top the soma

marwan-abdellah avatar Jan 13 '22 11:01 marwan-abdellah

Hi @marwan-abdellah , maybe it's unrelated, this is where I got the morphology from: http://neuromorpho.org/neuron_info.jsp?neuron_name=2017-16-05-slice-1-cell-6-rotated thanks

orena1 avatar Jan 13 '22 20:01 orena1