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A question about -X

Open NJU-Bio-Info opened this issue 2 years ago • 0 comments

Hi, when I use bowtie2 to perform CUT&Tag alignment. I set -X 700 and filter the reads whose mapq<20 but I can still observe fragment size >700 in bam file. I wonder why I get this result?

NJU-Bio-Info avatar Apr 26 '22 14:04 NJU-Bio-Info