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Select local alignment or end-to-end alignment for filtering rRNA

Open Duannai opened this issue 3 years ago • 0 comments

Hello, I have been working on the metatranscriptomics (2x150 bp), and I want to align the adapter-free and high-quality sequences to the Silva rRNA database, to remove the mapped rRNA reads.

Because the reference sequences is longer than those in metatranscriptomics, I think the local alignment should be better.

Could you please give me some advices?

Thanks in advance!

Mort

Duannai avatar Nov 28 '21 16:11 Duannai