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Updating the default V4 EMP primers

Open mestaki opened this issue 3 years ago • 1 comments

Just noticed that the V4 primers being used in the weight assembly scripts are using the old EMP primers (ex here). A few years back these were modified slightly and degeneracy added (as described here). I'm not sure if this would have any real significant effect but might be worth updating nevertheless.

Primers currently being used in weight assembly: FWD: GTGCCAGCMGCCGCGGTAA REV: GGACTACHVGGGTWTCTAAT

Recommended update to new EMP primers: FWD: GTGYCAGCMGCCGCGGTAA; REV: GGACTACNVGGGTWTCTAAT

mestaki avatar Oct 19 '21 17:10 mestaki

I agree, definitely worth updating!

The effect on weights would probably be minor, but the newer primers are presumably what most new studies would be using, so important to update.

nbokulich avatar Oct 20 '21 08:10 nbokulich