Austin Hartman

Results 24 comments of Austin Hartman

What's the full link giving you a 404 error? [https://seurat.nygenome.org/vignette_data/spatial_vignette_2/nanostring_data.Rds](https://seurat.nygenome.org/vignette_data/spatial_vignette_2/nanostring_data.Rds) appears to be working.

Hi, thanks for the PR. It looks like there is a bit of duplication with Read10x, so is it possible to use Read10x to load your data in (possibly after...

Hi, apologies for the slow reply - the bug in the `FOV` subset method should now be fixed on the `feat/imaging` branch.

Hi, thanks for raising this. Please see the latest commit of the feat/imaging branch to find a fix supporting underscores in fov names. Let me know if that solves your...

Hi, I am actually able to load in liver 1 slice 1. The warning suggests a lot of missing data - could you double check all of the cell boundary...

thanks for the suggestion - I agree this would be useful and I will update the issue once it's added. For the second point, it might be useful to plot...

Hi @cplaisier, I am having trouble reproducing on the latest version of Seurat using a [human melanoma dataset from 10x](https://www.10xgenomics.com/resources/datasets/human-melanoma-if-stained-ffpe-2-standard). Could you post your session info and possibly a publicly...

Hi, could you please post your `sessionInfo()` and a small reproducible example if possible so we can better assess whats going on?

Regarding the negative probes, I think an optional way to include them would be beneficial for QC. Negative control probe counts and negative control padlock counts can be stored as...

Hi, thanks for pointing out this issue. `MapQuery` uses the UMAP models from uwot to project query cells into the reference UMAP space and the latest uwot versions add a...