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Published 20 hours ago • verified publisher icon Adrian-Cantu

Fastq quality output

Open pbravakos opened this issue 4 years ago • 3 comments

Hi, I am running Prinseq-plus-plus on paired end fastq files and the ouput seems to be probematic. In at least one read (haven't checked all the reads) the lengths of sequence and quality values differ. I am pasting below the problematic read:

@V1:1:HYLGGDSXX:3:2678:28212:36761/1
TCTTTATTTAAACTTTTATATTTATAAAATTTATATTTACTAAAAAAATTTTTATTGTTAATTATTTTTTTAAAATTATTTTTATTAAATTTAATAATATTTTTAGACTCAAATTCTTTTAATAACCCAACATTA
+
FFFFFFFFFFFFFFFFFFFFFFFF,FF:FFFFFF

Do you have any idea why this could be happening? Thanks.

pbravakos avatar Jul 05 '20 09:07 pbravakos