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RELION 4.0's Manual picker launched from AutoPick results ignores the pixel size
This is because the new two-column coordinate STAR files do not have the pixel size. Thus the manual picker ends up using 1 A/px, leading to wrong low pass filtering and a wrong particle diameter. See https://github.com/3dem/relion/blob/ver4.0/src/manualpicker.cpp#L727.
This only affects rendering, not processing, but is confusing. A possible fix is to store the pixel size in the new coordinate STAR file. A user-side workaround is to explicitly specify the pixel size in a Manual Pick job and save the job setting.