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Fix for barcode reuse?

Open AMechels opened this issue 1 year ago • 2 comments

Dear developers,

I wanted to use your tool enclone on 2 bcr files from 1 donor, before and after treatment. However, I get following message:

Significant barcode reuse detected.  If at least 25% of the barcodes in one dataset
are present in another dataset, is is likely that two datasets arising from the
same library were included as input to enclone.  Since this would normally occur
only by accident, enclone exits.  If you wish to override this behavior,
please rerun with the argument ACCEPT_REUSE.

Here are the instances of reuse that were observed:

bcrPBMC_JUQ064_hg19, bcrPBMC_JUQ065_hg19 ==> 245 of 972, 829 barcodes (29.6%)

The barcodes don't have a prefix or suffix, so it's only normal that some are present in both. Is there an argument I can set that adds a string to the barcode using the META csv file?

Thanks in advance, Aurelie

AMechels avatar Jul 18 '22 16:07 AMechels