The genome size is too big

[rillaxy]

Dear Professor Zhang, The species I study is an insect which has 36 chromosomes,and its genome size is 5.6Gb. The initial genome was mounted to 40 chromosomes using Allhic software(Because the size of the last few chromosomes are too small ,so I set k=40). I try to convert the .fasta file obtained from Allhic to .hic .assemby by matlock and juicebox_script. Do you suggest that I directly operate this transformation on the whole genome or operate according to the per chromosome? Because my computer memory is limited(8Gb), I worry that the manual adjustment in the later stage will be very slow ? I just want to break down somewhere that's obviously wrong with the connection. Can you give me some suggestions,? Thanks for your listening, I'm looking forward to your reply! Rilla

[tangerzhang]

Hi @rillaxy I have very limited experience with juice box adjustment. And I have no idea if it works on a 5.6-Gb genome. A hic plot and dot plot analysis will provide an initial assessment of genome scaffolding. If the quality is ok, I think there is no need to use the juicebox for manual correction.

[rillaxy]

Thanks for your fast responses, I will try it. Rilla

[taotaoyuan]

I had the same problem. The computer wouldn't run. How did you solve it, juicer?

[rillaxy]

这是来自QQ邮箱的假期自动回复邮件。   您好，我已经收到您的邮件，祝生活幸福！