rnaseq
rnaseq copied to clipboard
Adding stubs at all level
- No stubs for
utils_*subworkflows (local or from nf-core as no process so no stubs). - modules/local:
- https://github.com/nf-core/rnaseq/pull/1331
- modules/nf-core:
- https://github.com/nf-core/modules/pull/5854
- https://github.com/nf-core/modules/pull/5861
- subworkflows/local:
- This current PR
- subworkflows/nf-core:
- https://github.com/nf-core/modules/pull/5911
- https://github.com/nf-core/modules/pull/5913
- https://github.com/nf-core/modules/pull/5921
- https://github.com/nf-core/modules/pull/5922
- pipeline:
- This current PR, with a crude implementation
PR checklist
- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add tests!
- [ ] If you've added a new tool - have you followed the pipeline conventions in the contribution docs
- [ ] If necessary, also make a PR on the nf-core/rnaseq branch on the nf-core/test-datasets repository.
- [ ] Make sure your code lints (
nf-core lint). - [ ] Ensure the test suite passes (
nextflow run . -profile test,docker --outdir <OUTDIR>). - [ ] Check for unexpected warnings in debug mode (
nextflow run . -profile debug,test,docker --outdir <OUTDIR>). - [ ] Usage Documentation in
docs/usage.mdis updated. - [ ] Output Documentation in
docs/output.mdis updated. - [ ]
CHANGELOG.mdis updated. - [ ]
README.mdis updated (including new tool citations and authors/contributors).
nf-core lint overall result: Passed :white_check_mark: :warning:
Posted for pipeline commit 1b34d1e
+| ✅ 173 tests passed |+
#| ❔ 9 tests were ignored |#
!| ❗ 7 tests had warnings |!
:heavy_exclamation_mark: Test warnings:
- files_exist - File not found:
assets/multiqc_config.yml - files_exist - File not found:
.github/workflows/awstest.yml - files_exist - File not found:
.github/workflows/awsfulltest.yml - pipeline_todos - TODO string in
main.nf: Optionally add in-text citation tools to this list. - pipeline_todos - TODO string in
main.nf: Optionally add bibliographic entries to this list. - pipeline_todos - TODO string in
main.nf: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - pipeline_todos - TODO string in
methods_description_template.yml: #Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline
:grey_question: Tests ignored:
- files_exist - File is ignored:
conf/modules.config - nextflow_config - Config default ignored: params.ribo_database_manifest
- files_unchanged - File ignored due to lint config:
assets/email_template.html - files_unchanged - File ignored due to lint config:
assets/email_template.txt - files_unchanged - File ignored due to lint config:
.gitignoreor.prettierignore - actions_ci - actions_ci
- actions_awstest - 'awstest.yml' workflow not found:
/home/runner/work/rnaseq/rnaseq/.github/workflows/awstest.yml - multiqc_config - multiqc_config
- modules_config - modules_config
:white_check_mark: Tests passed:
- files_exist - File found:
.gitattributes - files_exist - File found:
.gitignore - files_exist - File found:
.nf-core.yml - files_exist - File found:
.editorconfig - files_exist - File found:
.prettierignore - files_exist - File found:
.prettierrc.yml - files_exist - File found:
CHANGELOG.md - files_exist - File found:
CITATIONS.md - files_exist - File found:
CODE_OF_CONDUCT.md - files_exist - File found:
LICENSEorLICENSE.mdorLICENCEorLICENCE.md - files_exist - File found:
nextflow_schema.json - files_exist - File found:
nextflow.config - files_exist - File found:
README.md - files_exist - File found:
.github/.dockstore.yml - files_exist - File found:
.github/CONTRIBUTING.md - files_exist - File found:
.github/ISSUE_TEMPLATE/bug_report.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/config.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/feature_request.yml - files_exist - File found:
.github/PULL_REQUEST_TEMPLATE.md - files_exist - File found:
.github/workflows/branch.yml - files_exist - File found:
.github/workflows/ci.yml - files_exist - File found:
.github/workflows/linting_comment.yml - files_exist - File found:
.github/workflows/linting.yml - files_exist - File found:
assets/email_template.html - files_exist - File found:
assets/email_template.txt - files_exist - File found:
assets/sendmail_template.txt - files_exist - File found:
assets/nf-core-rnaseq_logo_light.png - files_exist - File found:
conf/test.config - files_exist - File found:
conf/test_full.config - files_exist - File found:
docs/images/nf-core-rnaseq_logo_light.png - files_exist - File found:
docs/images/nf-core-rnaseq_logo_dark.png - files_exist - File found:
docs/output.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/usage.md - files_exist - File found:
main.nf - files_exist - File found:
conf/base.config - files_exist - File found:
conf/igenomes.config - files_exist - File found:
modules.json - files_exist - File not found check:
.github/ISSUE_TEMPLATE/bug_report.md - files_exist - File not found check:
.github/ISSUE_TEMPLATE/feature_request.md - files_exist - File not found check:
.github/workflows/push_dockerhub.yml - files_exist - File not found check:
.markdownlint.yml - files_exist - File not found check:
.nf-core.yaml - files_exist - File not found check:
.yamllint.yml - files_exist - File not found check:
bin/markdown_to_html.r - files_exist - File not found check:
conf/aws.config - files_exist - File not found check:
docs/images/nf-core-rnaseq_logo.png - files_exist - File not found check:
lib/Checks.groovy - files_exist - File not found check:
lib/Completion.groovy - files_exist - File not found check:
lib/NfcoreTemplate.groovy - files_exist - File not found check:
lib/Utils.groovy - files_exist - File not found check:
lib/Workflow.groovy - files_exist - File not found check:
lib/WorkflowMain.groovy - files_exist - File not found check:
lib/WorkflowRnaseq.groovy - files_exist - File not found check:
parameters.settings.json - files_exist - File not found check:
pipeline_template.yml - files_exist - File not found check:
Singularity - files_exist - File not found check:
lib/nfcore_external_java_deps.jar - files_exist - File not found check:
.travis.yml - nextflow_config - Config variable found:
manifest.name - nextflow_config - Config variable found:
manifest.nextflowVersion - nextflow_config - Config variable found:
manifest.description - nextflow_config - Config variable found:
manifest.version - nextflow_config - Config variable found:
manifest.homePage - nextflow_config - Config variable found:
timeline.enabled - nextflow_config - Config variable found:
trace.enabled - nextflow_config - Config variable found:
report.enabled - nextflow_config - Config variable found:
dag.enabled - nextflow_config - Config variable found:
process.cpus - nextflow_config - Config variable found:
process.memory - nextflow_config - Config variable found:
process.time - nextflow_config - Config variable found:
params.outdir - nextflow_config - Config variable found:
params.input - nextflow_config - Config variable found:
params.validationShowHiddenParams - nextflow_config - Config variable found:
params.validationSchemaIgnoreParams - nextflow_config - Config variable found:
manifest.mainScript - nextflow_config - Config variable found:
timeline.file - nextflow_config - Config variable found:
trace.file - nextflow_config - Config variable found:
report.file - nextflow_config - Config variable found:
dag.file - nextflow_config - Config variable (correctly) not found:
params.nf_required_version - nextflow_config - Config variable (correctly) not found:
params.container - nextflow_config - Config variable (correctly) not found:
params.singleEnd - nextflow_config - Config variable (correctly) not found:
params.igenomesIgnore - nextflow_config - Config variable (correctly) not found:
params.name - nextflow_config - Config variable (correctly) not found:
params.enable_conda - nextflow_config - Config
timeline.enabledhad correct value:true - nextflow_config - Config
report.enabledhad correct value:true - nextflow_config - Config
trace.enabledhad correct value:true - nextflow_config - Config
dag.enabledhad correct value:true - nextflow_config - Config
manifest.namebegan withnf-core/ - nextflow_config - Config variable
manifest.homePagebegan with https://github.com/nf-core/ - nextflow_config - Config
dag.fileended with.html - nextflow_config - Config variable
manifest.nextflowVersionstarted with >= or !>= - nextflow_config - Config
manifest.versionends indev:3.15.0dev - nextflow_config - Config
params.custom_config_versionis set tomaster - nextflow_config - Config
params.custom_config_baseis set tohttps://raw.githubusercontent.com/nf-core/configs/master - nextflow_config - Lines for loading custom profiles found
- nextflow_config - nextflow.config contains configuration profile
test - nextflow_config - Config default value correct: params.hisat2_build_memory= 200.GB
- nextflow_config - Config default value correct: params.gtf_extra_attributes= gene_name
- nextflow_config - Config default value correct: params.gtf_group_features= gene_id
- nextflow_config - Config default value correct: params.featurecounts_group_type= gene_biotype
- nextflow_config - Config default value correct: params.featurecounts_feature_type= exon
- nextflow_config - Config default value correct: params.igenomes_base= s3://ngi-igenomes/igenomes/
- nextflow_config - Config default value correct: params.trimmer= trimgalore
- nextflow_config - Config default value correct: params.min_trimmed_reads= 10000
- nextflow_config - Config default value correct: params.umitools_extract_method= string
- nextflow_config - Config default value correct: params.umitools_grouping_method= directional
- nextflow_config - Config default value correct: params.aligner= star_salmon
- nextflow_config - Config default value correct: params.pseudo_aligner_kmer_size= 31
- nextflow_config - Config default value correct: params.min_mapped_reads= 5.0
- nextflow_config - Config default value correct: params.kallisto_quant_fraglen= 200
- nextflow_config - Config default value correct: params.kallisto_quant_fraglen_sd= 200
- nextflow_config - Config default value correct: params.stranded_threshold= 0.8
- nextflow_config - Config default value correct: params.unstranded_threshold= 0.1
- nextflow_config - Config default value correct: params.deseq2_vst= true
- nextflow_config - Config default value correct: params.rseqc_modules= bam_stat,inner_distance,infer_experiment,junction_annotation,junction_saturation,read_distribution,read_duplication
- nextflow_config - Config default value correct: params.skip_bbsplit= true
- nextflow_config - Config default value correct: params.skip_preseq= true
- nextflow_config - Config default value correct: params.custom_config_version= master
- nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
- nextflow_config - Config default value correct: params.max_cpus= 16
- nextflow_config - Config default value correct: params.max_memory= 128.GB
- nextflow_config - Config default value correct: params.max_time= 240.h
- nextflow_config - Config default value correct: params.publish_dir_mode= copy
- nextflow_config - Config default value correct: params.max_multiqc_email_size= 25.MB
- nextflow_config - Config default value correct: params.validate_params= true
- nextflow_config - Config default value correct: params.pipelines_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/7f1614baeb0ddf66e60be78c3d9fa55440465ac8/
- files_unchanged -
.gitattributesmatches the template - files_unchanged -
.prettierrc.ymlmatches the template - files_unchanged -
CODE_OF_CONDUCT.mdmatches the template - files_unchanged -
LICENSEmatches the template - files_unchanged -
.github/.dockstore.ymlmatches the template - files_unchanged -
.github/CONTRIBUTING.mdmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/bug_report.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/config.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/feature_request.ymlmatches the template - files_unchanged -
.github/PULL_REQUEST_TEMPLATE.mdmatches the template - files_unchanged -
.github/workflows/branch.ymlmatches the template - files_unchanged -
.github/workflows/linting_comment.ymlmatches the template - files_unchanged -
.github/workflows/linting.ymlmatches the template - files_unchanged -
assets/sendmail_template.txtmatches the template - files_unchanged -
assets/nf-core-rnaseq_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-rnaseq_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-rnaseq_logo_dark.pngmatches the template - files_unchanged -
docs/README.mdmatches the template - readme - README Nextflow minimum version badge matched config. Badge:
23.04.0, Config:23.04.0 - readme - README Zenodo placeholder was replaced with DOI.
- pipeline_name_conventions - Name adheres to nf-core convention
- template_strings - Did not find any Jinja template strings (560 files)
- schema_lint - Schema lint passed
- schema_lint - Schema title + description lint passed
- schema_lint - Input mimetype lint passed: 'text/csv'
- schema_params - Schema matched params returned from nextflow config
- system_exit - No
System.exitcalls found - actions_schema_validation - Workflow validation passed: branch.yml
- actions_schema_validation - Workflow validation passed: fix-linting.yml
- actions_schema_validation - Workflow validation passed: linting.yml
- actions_schema_validation - Workflow validation passed: cloud_tests_small.yml
- actions_schema_validation - Workflow validation passed: clean-up.yml
- actions_schema_validation - Workflow validation passed: ci.yml
- actions_schema_validation - Workflow validation passed: linting_comment.yml
- actions_schema_validation - Workflow validation passed: download_pipeline.yml
- actions_schema_validation - Workflow validation passed: release-announcements.yml
- actions_schema_validation - Workflow validation passed: cloud_tests_full.yml
- merge_markers - No merge markers found in pipeline files
- modules_json - Only installed modules found in
modules.json - modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
- base_config -
conf/base.configfound and not ignored. - nfcore_yml - Repository type in
.nf-core.ymlis valid:pipeline - nfcore_yml - nf-core version in
.nf-core.ymlis set to the latest version:2.14.1
Run details
- nf-core/tools version 2.14.1
- Run at
2024-08-21 08:20:39
hitting:
-[nf-core/rnaseq] Pipeline completed successfully with skipped sampl(es)-
-[nf-core/rnaseq] Please check MultiQC report: 5/5 samples skipped since they failed 10000 trimmed read threshold.-
with stub level pipeline tests...
Related issues and PRs:
- https://github.com/nf-core/tools/issues/3049
- https://github.com/nf-core/website/pull/2621
New error coming out of Picard:
A field field parsed out of a read name was expected to contain an integer and did not. Read name: SRR6357072.6014972. Cause: String 'SRR6357072.6014972' did not start with a parsable number.
Picard/markduplicates is held back to 3.1.1 due to failing tests at the pipeline level cf https://github.com/nf-core/rnaseq/issues/1346
Please update the changelog before any merge
Please update the changelog before any merge
CHANGELOG updated