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option to split pod5 by size/read number

Open olawa opened this issue 1 year ago • 3 comments

Hi,

I have used pod5 convert fast5 and as a result have only a single pod5 per run. It would be more practial to have them split into files with a fixed read number or bases, is this possible with any of the filter/subset commands?

olawa avatar Feb 04 '24 16:02 olawa

Hi @olawa ,

The convert tool has a the --one-to-one option mentioned in the documentation where a pod5 file is created per fast5 if this is the file arrangement you prefer (approx 4k reads per file).

The pod5 subset tool can be used to split files by any mapping that you want.

Kind regards, Rich

HalfPhoton avatar Feb 05 '24 10:02 HalfPhoton

@olawa @HalfPhoton - hopefully #93 has been fixed in current version of pod5 subset ...

sklages avatar Feb 07 '24 08:02 sklages

Hi @sklages, unfortunately not - I've had to pause that work for a little while. Hopefully I'll have time to back to it soon and I'll let you know when we get something out.

HalfPhoton avatar Feb 07 '24 11:02 HalfPhoton